Workflow needs

Some of the most pressing workflow needs are in the early stages of cell line development, where there is pressure to provide early-phase clinical material as quickly as possible. Here it is important to reduce timelines to a minimum.

The key to accelerating the workflow is choosing the right technology. The table below gives an summary of these workflow needs and indicates the role of the Cell Metric CLD™.

Overall, by employing Cell Metric CLD™ in the upstream workflow, up to 25% of time can be saved in the cell line development process.

Workflow needsBenefits of Cell Metric CLD™
Screen 10s or 100s of typically 96-well plates to find wells containing single cells or colonies derived from single cells
  • Fast read times (<3 minutes per plate)
  • Images the whole well
  • Temperature controlled stacker for batch of 10 lidded plates
  • Audit trail tracks plates and wells for full history
Find wells that came from a single cell, and as quickly as possible
  • Highest-quality image means you can see single cells straight after seeding
  • Images right up to and into edges of wells so no cells are missed
  • Provides photo-evidence proof of monoclonality
  • Avoids subcloning (saves weeks of time)
Measure growth and stability of clones
  • Automatically measures cell number and plots growth rate for each clone
  • Unlimited number of data points for each growth curve
Work with different plate formats to support clone screening, media optimisation and scale-up
  • Works with all plate formats including 384-,  96-, 24- and 6-well
Get cells from static culture to liquid culture as quickly as possible
  • Supports workflows using suspension cells in liquid media
  • Time saved by avoiding subcloning
Avoid fluorescent labels, which can affect cell growth, viability, gene expression and, importantly, compliance with regulations for biotherapeutic use
  • High-resolution brightfield imaging of single cells means fluorescence is not required for cell imaging
Accurate workflow management for large numbers of clones
  • Tracks images and growth metrics for thousands of wells
  • Keeps tabs on your most promising clones
  • Export of data for downstream applications such as liquid handling

 

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