Clarity in cell line development

Single cell cloning of FACS-sensitive cells using the VIPS

In this case study, Celonic AG showed the impact of switching from a LD workflow to one which combined FACS + VIPS. Although FACS was useful for enrichment, it damaged their CHO cell line for single cell seeding resulting in poor survival and outgrowth.

By implementing the VIPS for gentle single cell cloning combined with cloning media optimisation, outgrowth improved from around 5% with FACS to over 60% using the VIPS.

These changes benefited Celonix by reducing the cloning plates per project by up to 25-fold.

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