High efficiency single cell cloning of iPSCs using combination of MatriClone™ and VIPS™
As the interest in the therapeutic value of iPSCs accelerates, so does the need to establish the regulatory framework and best practice in this area.
In this application note, Solentim introduces a novel workflow using the high efficiency single cell seeder VIPS™ and a new soluble matrix called MatriClone™ designed to support single cell growth and subsequent outgrowth.
Higher Viability - Improved Outgrowth
Starting with enhanced seeding efficiency and gentle seeding from VIPS and adding new levels of growth support with MatriClone, this new workflow demonstrates enhanced performance over current methodologies and matrices whilst maintaining stem cell pluripotency.
Assurance of Clonality
The VIPS system offers an industry-accepted ‘double lock’ methodology to record quality evidence of clonality. This data, wrapped up in a clonality report, is designed to meet the needs of Regulators when they review IND applications for new therapeutics.
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