For use at either static or expansion stages, ICON Titer Plates offers a rapid plate-based assessment of IgG concentration. The plate is in 96-well format and is supplied pre-coated with an anti-FC fluorescent probe ready for use. The plate is designed for assessment on the ICON instrument using its fluorescence polarization reader.
Each well requires 60ul sample/pre-diluted sample. Two plate types are available. A ‘low’ range version measures a final concentration of 2.5 – 100 mg/L and a ‘PLUS’ range that measures 100 – 1500 mg/L.
Figure 1. ICON Titer plates are precoated and available in 96 well format.
ICON Titer uses the fastest and easiest IgG assay available, fluorescence polarization (FP). FP is a well-documented naturally occurring phenomenon describing rotational diffusion, namely the observation that large complexes rotate slowly in solution and small complexes rotate more rapidly.
Plates are supplied pre-coated with an IgG-specific fluorescent probe. After a short incubation with supernatant or cell suspension, the plate is illuminated by polarized light. Where the probe binds to IgG, a large complex will form and relates to a reduction in emitted polarized light. This difference in rotation rate and thus in emitted light is compared against a standard curve, providing an accurate concentration quantification (see figure 2).
Figure 2: ICON uses fluorescent based polarization assay to quantify IgG concentration in solution.
ICON Titer plates are supplied sterile and welded in gas-permeable packaging.
- Shipping conditions: Chilled
- Storage conditions: Refrigerated (2-8°C)
- Shelf life: 18 months
- RS-3221 ICON Titer Assay Plate - Plate based IgG assay for cloning plates. Five plates, each for 96 samples. For 2.5 mg/L - 100 mg/L.
- RS-3223 ICON Titer PLUS Assay Plate - Plate based IgG assay. Five plates each for 96 samples. For 100 mg/L - 1500 mg/L.