In this poster we use Horizon Discovery’s HD BIOP3 GS Knockout CHO KI cell line in combination with the VIPS seeding and optimised outgrowth conditions using SAL Scientific InstiGRO CHO reagents to produce a significantly higher number of colonies per plate, thereby reducing the number of plates needing to be screened.

The experiment was designed to assess the outgrowth of the HD-BIOP3 cell line by manual limiting dilution and VIPS under a variety of conditions.