From limiting dilution, to seeders, pickers and imagers, Mark discusses validation techniques for clonally-derived workflows best practices in this area
“How do I go about validating an image based workflow?”
- Understanding single cells, the statistical and the real-life.
- ‘Probability’ versus ‘quality of evidence’.
- Understanding error rates in FACS and cell-printing methods and how imaging is needed to ‘rescue’ the situation.
- Best practices for validation methodologies.
Mark Stockdale discusses image-based workflows with Duncan Borthwick