Cell Metric® CLD

Cell Metric® CLD is our premium system with built-in plate loader for 10 plates

Cell Metric CLD

Groups that benefit from this system typically have larger projects or multiple projects. The Cell Metric CLD includes an integrated, temperature-controlled plate loader. A batch of up to 10 lidded plates is loaded manually into a cassette which is placed into the stacker and the plates are automatically transferred for imaging one at a time with barcode reading (watch video of how the plate loader works). Additional cassettes can be purchased. (add an image of plate loader/cassette as used in the brochure)

The Cell Metric CLD uses non-invasive bright field imaging for analysis of settled suspension or adherent cells. Cells are imaged in lidded, flat-bottomed microplates (typically 96 or 384 well format) in their normal liquid media environment.

Scanning of the entire plate is rapid (approx. 4-5 minutes) with whole-well images captured automatically at single-cell resolution (insert and image of Day 0 and Day 1 – use the ones from the home page montage).  Focus of all wells in the plate is 100% guaranteed which largely eliminates user misclassification of wells and subsequent ghost wells. A built-in barcode reader ensures accurate tracking of plates.

Cell Metric Day 0 Image

Cell Metric Day 2 Image

Results are reviewed at the instrument computer or alternatively at your desktop using the optional remote viewer software. Image data can be shared between different groups or geographical sites using the remote viewer software. Colonies are tracked back in the image library for an individual well to confirm a single cell starting point (see images below).

Cell Metric Colony

Cell Metric 2 Cells

Cell Metric 1 Cells

For clonal wells a hit picking file can be generated (as .CSV file) and exported to third-party liquid handling robots. Such automated picking is particularly useful where using 384 well plates.

Cell Metric CLD comes provided with the Clonality Report software which is a documentation package for the clones of interest. This report includes all the specific requirements from the FDA guidance (whole well image, location of single cell feature, identification of debris etc) for Day 0 and subsequent time points along with full audit trail of those clones. Once wells and time points have been selected, a report is automatically generated as a PDF or Powerpoint. This report can be included as part of an IND submission, given to the cell banking group or provided to a CRO client as part of the project handover.

Clonality Report

The Cell Metric CLD can also be purchased with an option to Verify Clonality via Fluorescence. This is two-colour (red/green) fluorescence detection which can enable the user to validate a new a cloning method or quality check their existing method.

For lower-throughput requirements, see Cell Metric

Key metrics measured by Cell Metric

Metric measuredDescriptionGraphic representation
Assurance of clonality Single cell resolution image of whole well on Day 0, and image library of subsequent days Clonality Report
Cell number A direct count of the number of cells per well Cell numbers per well
Confluence Measures percentage coverage or carpeting of the well base Confluence % for each well
Growth rates and Doublings Uses the cell count to generate and automatically update (using plate and well ID) growth curves for each clone Growth curves
Cloning Method Efficiency (Option) Requires option for Verify Clonality via Fluorescence Plate overview map of wells with the fluorescent object count (for generating statistics)