Next Generation Cell Culture Supplements

SolentimProductsNext Generation Cell Culture Supplements

Enhancing Cell Line Development Workflows

In the search for high-value cells, growth conditions must be optimized to support early growth conditions.

Cell growth and viability can be impaired in defined, animal component-free media, especially when subjected to stress.

High producer cells are more sensitive to media conditions and are likely to be at a growth disadvantage when compared to non-producers.

Comparing outgrowth of CHOZN® cells selected through manual limiting dilution versus VIPS  and InstiGRO, the VIPS/InstiGRO shows an approximate seven-fold improvement in total outgrowth per plate.

Introducing InstiGRO™, InstiSHAKE™ and InstiTHAW™

  • Certified Animal-Free
  • Manufactured under ISO 9001

Insti Cell Culture Supplements Accelerate growth

High Performance Cell Growth Supplements

Advanced cell culture supplements for use with Chinese hamster ovary (CHO) and human embryonic kidney (HEK) cells.
Solentim Cell Culture Supplements Workflow

 

The Insti range of supplements is designed to enhance growth at different stages of cell line development for accelerated workflows:

- InstiGRO™ to assist with early cell growth and single cell survival

- InstiSHAKE™ as a follow on supplement to InstiGRO in the transition to deep well plates

- InstiTHAW™ to protect cells during freezing and thawing.

VIPS™ + InstiGRO™: a powerful combination

Verified In-situ Plate Screening (VIPS™) is our high-efficiency, single-cell seeder. This low-pressure system demonstrates higher seeding efficiency and outgrowth when compared to limiting dilution methods and provides a double-lock of assurance with enhanced in-well imaging to track seeding and clonal growth.

Complementing our VIPS technology, cell outgrowth is further enhanced with the use of InstiGRO™ and InstiGRO™ PLUS cell growth supplements.

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Viral Vector Production for Gene Therapy – the benefits of using InstiGRO HEK

GSK report InstiGRO HEK had a significant positive effect on clonal outgrowth in all conditions tested, with increase in outgrowth ranging from a factor of 2.7 to 11.5

Cell Line Development using InstiGRO CHO

When comparing outgrowth of CHOZN® cells selected through limiting dilution versus the VIPS +/- InstiGRO CHOPLUS method, VIPS + InstiGRO CHOPLUS shows an approximate seven-fold improvement in total outgrowth per plate.

Ordering information

 

InstiGRO Product Name Product Size (ml)
RS-1105 InstiGRO CHO 5
RS-1125 InstiGRO CHO 25
RS-1205 InstiGRO CHOPLUS 5
RS-1225 InstiGRO CHOPLUS 25
RS-1305 InstiGRO HEK 5
RS-1325 InstiGRO HEK 25
InstiSHAKE Product Name Product Size (ml)
RS-2125 InstiSHAKE CHO Sol A - 20  Sol B - 5
RS-2325 InstiSHAKE HEK 25
InstiTHAW Product Name Product Size (ml)
RS-3105 InstiTHAW CHO 5
RS-3305 InstiTHAW HEK 5

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