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Soluble Matrix for High Efficiency, High Viability iPSC Single Cell Cloning and Outgrowth

MatriClone™ is a new matrix designed to support iPSC growth in single seeding workflows while maintaining pluripotency. MatriClone is optimised for ‘in-solution’ seeding and optically suited for automated imaging.

When used as part of the VIPS seeding protocol, MatriClone offers high levels of single cell survival and subsequent outgrowth, all in a simple, automated workflow which avoids the need for coating plates. MatriClone is a defined matrix consisting of a recombinant peptide and is animal component-free.

A New Standard For iPSC Support

Why the need for change? Existing methodologies can include the likes of MatriGel, a chemically undefined mixture secreted from mouse sarcoma cells which must be manually coated onto plates.

Often used with limiting dilution, a low efficiency statistical approach to single cell seeding, this approach is time-consuming and lacks in evidence of clonality.

As demonstrated in this new application note, MatriClone offers a 2-3 fold increase in confluency when compared to MatriGel and VIPS a further 2-3 fold increase over limiting dilution.

Designed For Use With Automated Imaging

MatriClone offers ideal optical quality to contribute to high quality data when imaged by automated imaging platforms.

This makes MatriClone ideally suited to workflows using post-seeding technologies including the Cell Metric® whole well imager.

 

Comparing VIPS/MatriClone stem cell culture medium with LD

Colony outgrowth image captured on the VIPS at day 14 illustrating the enhanced number of colonies from VIPS seeded plates compared with limiting dilutions (LD).

 

Maintenance of Phenotype, Karyotype and Pluripotency

Importantly, clones generated using the VIPS + MatriClone workflow have been shown by immunocytochemistry and karyotyping to maintain pluripotency after at least 10 passages and have retained their ability to successfully differentiate.

VIPS iPSC Seeding and outgrowth

Ordering information

 

Product Code Product Name Description
RS-2100 MatriClone -  2x175ug Soluble recombinant peptide matrix for coating plates and adaptation of iPSC prior to single cell seeding on the VIPS. 2 vial pack (175ug/vial)
For RUO
Note - a vial is sufficient for 20 x 96 well plates
RS-2101 MatriClone -  6x175ug Soluble recombinant peptide matrix for coating plates and adaptation of iPSC prior to single cell seeding on the VIPS/ 6 vial pack (175ug/vial)
For RUO
Note - a vial is sufficient for 20 x 96 well plates
A clinical-grade version of the matrix can be sourced on a request-only basis
  • Supplied sterile with Certificate of Analysis
  • Endotoxin-free
  • Mycoplasma-free

 

Products utilize Matrixome technology developed by Professor Kiyotoshi Sekiguchi.
This product is not available for sale in Japan, Taiwan and Korea.

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