Verified In-Situ Plate Seeding (VIPS™) takes single cell cloning and image based clonality assurance to a whole new level of quality. The multi-tasking, compact instrument delivers a rock solid, ‘double lock’ solution for regulatory submission.
VIPS accelerates projects. High seeding efficiency for both 96 and 384 well plates, single round workflows for massively enhanced productivity and reduced timelines.
With VIPS you get healthier cells, more confirmed single cells per plate and better outgrowth.
Two-round methodologies are a thing of the past.
Operating at a pressure of less than one psi, VIPS gently dispenses cells in nanoliter droplets into a plate well. Crucially, the cell is imaged in the bottom of the dry well as evidence of seeding. Automated imaging in multiple tight z-stack layers of the whole droplet commences with intelligence-based image analysis confirming the existence of a single cell. The well is then filled automatically with media.
Seeding for high clonal efficiency
For 96 and 384 well plates and quality single-project use consumables, we employ the simplest and safest method of single-cell dispensing, with no complex fluidics or mixed samples. VIPS uses low pressure to seed cells in nanoliter droplets into empty wells.
Always in focus
VIPS performs total plate laser focus scanning, adjusting for plate contours, to ensure an optimal focal position for each well.
Intelligent Image Analysis
Using advanced image analysis technology, z-stack images are processed to confirm the presence of a single cell.
In-line media dispensing
Immediately after confirmation of cell seeding, VIPS automatically dispenses culture media into the well, protecting your high-value cell.
QC verification process
To assist in the verification of your clonality workflow, VIPS offers a verification methodology option based on rare-event fluorescence imaging.
In-well z-stack imaging
Immediately after seeding, the droplet is imaged at 20 different focal positions. This broad z-stack encompasses the optimal plane for image analysis enabling confident single cell detection.
A single platform for both seeding and outgrowth
A seeder and a separate imager?
VIPS is a 2 in 1 system combining high efficiency seeding with high contrast whole well imaging, saving lab space and resources.
Confident submissions with automated clonality report
To complete the story, a clonality report is generated, cataloguing both in data and images the whole story from single cell cloning to colony. The VIPS clonality report can be directly used as an important source of evidence, with a strong track record of IND submission success.
Assurance based on evidence, not probability
Manual limiting dilution methodologies are based on pipetting deliberately low cell/well concentrations to boost the probability of a single cell. With the advent of VIPS and image based assurance, credible evidence is gathered at multiple time points.
VIP’s unmatched imaging for single cell clarity
Designed specifically for the task, Solentim technology is honed for single cell imaging in the droplet by way of z-stack imaging and in the whole well. With high clarity images, even when near the cell wall, VIPS generates confidence for the lab and the regulator.
VIPS is the Perfect Choice for
- Cell line development (CLD) for biologics
- Gene and cell therapy
- Human and induced pluripotent stem cells
Download a Case Study
and discover our customers' experiences with VIPS.
"For the VIPS, there's so little media centered in the well that it makes it easy to locate the cell and prove the health authorities that the cell is clonal"
Thomas Kelly, Scientist at Janssen R&D LLC
"Demonstrating monoclonality has never been more reliable and straightforward. No more ambiguity, no more ghost cells. Both our clients and our own scientists are very pleased with the data the VIPS instrument generates"
Ferenc Boldog, Director of Cell Line Development, ATUM