An application note covering potential workflow approaches to cloning method qualification and quality control using the new fluorescence Cell Metric application.
A key application for the Cell Metric® is to establish the clonal origin of colonies during cell line development. This application explains how the Cell Metric® can fit in to a cell line development workflow to meet this need.
An important application for the Cell Metric® is to monitor clone outgrowth using confluence after single cell seeding, and to monitor colony formation as part of a clone selection process. This application explains how the Cell Metric® can fit in to a cell line development workflow to meet this need.
This application note demonstrates how the Cell Metric® is able to greatly improve the traditional hybridoma approach for generating new antibodies as a precursor to stable cell line development.