Traditional methods of cell line development rely on screening thousands of clones to identify just a few high producers, commonly relying on fluorescent screening. This approach is outdated and has been superseded.

Using clever vector design and targeted gene integration, it is possible to create stable populations, with high, predictable expression of the GOI.

The key to this step change is transposases, which shift clonal distribution massively towards high producing clones, as shown in the figure below.