Single cell seeding

Single cell seeding

The requirements are to dispense single cells from a population into wells of microtiter plates.

This should be done at high efficiency so that most wells contain a single cell and they need to be viable so they grow into a colony over the next few days.

Cell types are predominantly CHO suspension cells which are expressing an antibody or recombinant protein of therapeutic importance.

To date, the common methods for seeding single cells and selecting colonies have been the following:

  • Limiting Dilutions
  • FACS
  • Picking from semi-solid media
  • Newer microfluidics and opto-electronic approaches

Solentim have now developed a system called the VIPS as the potentially preferred approach for cell line development groups.

The VIPS will generate a resultant plate with high cloning efficiency in around 10 minutes and due to the gentle nature of the dispensing method, most of these wells will go on and grow up successfully.

Single cell seeding

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