Screening to select for manufacturing

Clones which have been shown to be monoclonal, are adapted to serum-free media and grown in 24 DWP suspension culture.
Clones are screened and ranked for virus yield.

Finally, clones are subject to scale down models (shake flasks or ambr) at higher cell densities and screening for best media and culture conditions as a predictor for manufacturing conditions.

Top 3-4 clones will be selected for genetic stability studies, bioreactor yield and vector quality assessments.